Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

نویسندگان

  • Kazuki Kato
  • Yuhkoh Satouh
  • Hiroshi Nishimasu
  • Arisa Kurabayashi
  • Junko Morita
  • Yoshitaka Fujihara
  • Asami Oji
  • Ryuichiro Ishitani
  • Masahito Ikawa
  • Osamu Nureki
چکیده

Sperm-egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface. Whereas other FRs bind and uptake folates, JUNO binds IZUMO1 and establishes the cell-cell adhesion. However, the mechanism of IZUMO1 recognition by JUNO has remained elusive. Here we report the crystal structure of mouse JUNO, at 2.3 Å resolution. A structural comparison of JUNO with the FRs revealed that JUNO and the FRs have similar overall structures, but JUNO lacks the folate-binding pocket, thereby explaining the inability of JUNO to bind folate. Further complementation of Juno knockout eggs with mutant Juno messenger RNAs revealed that the conserved, surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. Our structure-based in vivo functional analyses provide a framework towards a mechanistic understanding of mammalian gamete recognition.

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عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2016